It was Rashford's second goal in as many games here following a barren run of matches that saw him go 11 without scoring.
The injury cut short his game time, with the 23-year-old playing only a limited role with England during their run to the Euro 2020 final in July.
Rashford has returned to the club's Carrington training ground to continue his recovery from a recent shoulder operation, manager Ole Gunnar Solskjaer said.
Rashford, who has yet to start a game at the tournament, picked up the injury in November while playing for Manchester United.
Rashford says he was racially abused on social media following Manchester United's defeat in the Europa League final.
Solskjaer said Rashford, 23, was available for the trip to Spain but might not be risked for the entire match.
Manchester United climbed two places up to second in the Premier League after Rashford scored deep into stoppage time to give them a 1-0 win over Wolverhampton.
Rashford is drawing interest from some of Europe’s top clubs but the young Manchester United forward said he wants to be a one-club man.
Rashford could miss Saturday’s Premier League match against West Ham United due to a shoulder injury, manager Ole Gunnar Solskjaer said.
Leeds United players have donated 25,000 pounds to a campaign providing free school meals to children in Britain after being inspired by Rashford’s efforts.
Rashford can become a “world class” forward if he sharpens his poacher’s instincts in front of goal, former defender Rio Ferdinand has said.
Footballer Marcus Rashford is among those to receive awards in Queen Elizabeth’s birthday honours.
Rashford says the task of reeling in Liverpool and Manchester City adds spice to Manchester United's bid to re-establish themselves as League title contenders.
Manchester United’s winger Marcus Rashford addressed how Jose Mourinho’s managerial spell United helped him develop as a football player.
In a painful blow to Manchester United manager Gunnar Solskajer, his player Marcus Rashford was confirmed as absent from Liverpool clash on Sunday because of his injury in the Red Devils victory over Wolves Wolverhampton last Wednesday.
Manchester United manager Ole Gunnar Solskjaer is determined to keep Paul Pogba and Marcus Rashford at Old Trafford, saying the pair are vital to his plans of building a team "worthy of the history of the club".
Manchester United boss Ole Gunnar Solskjaer says Marcus Rashford has the potential to be as good a centre-forward as England teammate Harry Kane as the pair prepare to face off in the Premier League this weekend.
Rashford will be hopeful of a starting spot in Southgate's team after impressing in the warm-up games, including a long-range goal against Costa Rica.
Rashford, 20, has been on the bench 15 times in the league this season but scored twice to secure a 2-1 victory over Liverpool on Saturday.
Marcus Rashford struck twice as Manchester United won the 200th North West derby with a 2-1 victory over Liverpool at Old Trafford on Saturday to open up a five point gap on their third-placed rivals.