Luis Enrique’s side top Group B with 10 points from five games, one point ahead of Sweden who have two matches in hand.
Busquets has been allowed to rejoin the squad, the Spanish football federation confirmed.
Spain start against Sweden in Group E on Monday night and it is possible some players will now feel the side-effects of the vaccination.
Barcelona star Lionel Messi revealed on Saturday that Pep Guardiola and Luis Enrique played an important role in his career with Barcelona.
Ramos missed two penalties but teammate Gerard Moreno came off the bench to grab a late equalizer and snatch a 1-1 draw away to Switzerland.
Luis Enrique has handed Atletico Madrid midfielder Marcos Llorente a first call-up and recalled Koke and Alvaro Morata to the squad.
Adama Traore was named in the Spain squad by coach Luis Enrique on Friday, three days after also being called up by Mali.
Germany plays in Group D of UEFA Nations League A alongside Switzerland, Ukraine and Spain.
Bartra has known Enrique since 2009 as he has worked under Enrique's command at Barcelona second team for two years.
Famous for their tiki-taka possession-based passing game, Spain struggled at this summer's World Cup.
The Spanish Football Association announced Luis Enrique as the new head coach of the Spanish national team on Monday.