Tuchel, who took over from sacked Julian Nagelsmann in late March, has now seen his team crash out of two competitions in two weeks after they were also knocked out of the German Cup by Freiburg in the last eight.
It was City's 15th consecutive game without defeat across all competitions and Pep Guardiola's team, losing finalists in 2021, will next face holders Real Madrid in the last four.
With the match being broadcast to 200 territories globally, all eyes will be on the former Paris St Germain and Chelsea coach who took over after Julian Nagelsmann's shock sacking days after Bayern's 2-1 loss at Bayer Leverkusen on March 19.
"It's a big disappointment," Galtier said. "We have to deal with it and accept it. There's a lot of disappointment in the dressing room."
Galtier added that he plans to fill Neymar's place with a midfielder instead of another attacker, as he did in last week's 3-0 win over rivals Olympique de Marseille, where Lionel Messi and Kylian Mbappe led the team to a second league win in a row.
Messi played the full 90 minutes of PSG's 2-1 defeat to Olympique Marseille in the French Cup on Wednesday but later felt pain in his hamstring, the report said.
Cancelo, a two-time championship winner with City who was selected twice in the Premier League Team of the Year, moved to Bayern on an initial loan deal and the Bundesliga side can sign the 28-year-old permanently in the close season.
Neuer was ruled out for the rest of the season after undergoing surgery on a broken bone following a skiing accident.
The 25-year-old tested positive during the World Cup in Qatar, which forced him to miss Morocco's 1-0 quarter-final victory over Portugal.
Jamal Musiala scored once and set up two more goals as champions Bayern Munich cruised past Bayer Leverkusen 4-0 on Friday to snap a four-game winless run in the Bundesliga and climb into second place.
Bayern Munich's four-game winless run -- their longest in the Bundesliga in 20 years -- has soured the mood among club bosses and piled pressure on coach Julian Nagelsmann to quickly turn things around.
The French defender is Sevilla's fourth signing of the transfer window after Marcao, Alex Telles and Isco.
The Bavarians put down a marker at the start of the campaign as they look to land an 11th consecutive league crown.
The DFL had predicted a drop in turnover of about one billion euros during the 2020-21 season if fans had to stay away throughout the campaign.
Dortmund start the weekend second in the table and nine points behind runaway Bundesliga leaders Bayern Munich, but with a game in hand.
With 23 league goals so far this season, Lewandowski remains on course to finish as the Bundesliga's top-scorer for the fifth year running.
The 25-year-old joined Bayern from Juventus in 2015, initially on a two-year loan, and went on to score the winning goal in the 2020 Champions League final.
Mueller celebrated his 400th Bundesliga appearance with a goal and an assist as the champions crushed VfL Wolfsburg 4-0 on Friday.
Barcelona crashed out of the Champions League following a 3-0 defeat at Bayern Munich on Wednesday, failing to make it past the group stage of the competition.
Barca, second in Group E behind Bayern, are due to face the German side on Dec. 8 at Allianz Arena.