Europa League kings Sevilla ignored the heavy rain and the pressure of the Italian crowd at a packed Allianz Stadium to dominate the game and looked well on course continue their bid for a record-extending seventh title before Gatti's last-gasp effort left the tie evenly poised.
They will meet Sevilla in the semi-finals, with the Italians at home first on May 11 before the return leg in Spain on May 18, after the Spanish side knocked out Manchester United 5-2 on aggregate.
Juventus took a small step towards the last four of the Europa League as Federico Gatti's second-half goal secured the hosts a 1-0 victory over Sporting Lisbon in the first leg of their quarter-final on Thursday.
The Tribuna Sud section of Juve's Allianz Stadium will be closed for their game against Napoli on April 23 after racist chants were heard coming from that area during the 1-1 first leg draw against Inter.
The French World Cup winner rejoined Juventus in July 2022 following his departure from Manchester United, and has had less than 40 minutes of playing time, coming on as a substitute against Torino and AS Roma in Serie A.
Chiesa was substituted in the second half, giving Allegri another concern before his team host Sampdoria in Serie A on Sunday.
The hosts got a deserved breakthrough just before halftime when Bremer perfectly timed a run into the box to connect with a cross from Filip Kostic and head the ball into the net.
With 20 games left to play this season, Juve were third in Serie A with 37 points, 10 behind leaders Napoli. The deduction pushes them down into 10th place, outside the qualifying spots for lucrative European competition.
Napoli moved 10 points clear at the top of the standings after snapping Juventus's eight-match Serie A winning streak.
Napoli are still top of the table with 41 points after 16 games but their advantage over second-placed AC Milan, who beat Salernitana 2-1, narrowed to five points.
Juventus manager Massimiliano Allegri said the team is in a positive mood after beating local rivals Torino in the last game but warned his players to be careful against a "fast and technical" Empoli side in Friday's Serie A match.
Juventus have lost their opening two games of the Champions League group stage for the first time and are sitting eighth in Serie A, four points behind leaders Napoli after six games.
Allegri's defensive-minded approach did not go down well with fans, who were also frustrated that the team faltered during games they had been dominating.
Vlahovic scored 31 seconds into his Champions League debut as Juventus drew 1-1 at Villarreal in the opening match of their round-of-16 tie.
The 25-year-old joined Bayern from Juventus in 2015, initially on a two-year loan, and went on to score the winning goal in the 2020 Champions League final.
With Malmo guaranteed to finish bottom of the group, the Swedes had little incentive to push for a leveller and Juve closed out the win without ever being seriously troubled.
In the following lines, ET sheds light on the most important world events that took place on November 1.
Allegri's side took down the European champions with a disciplined performance that sucked the life out of the Blues.
Five South American Juventus players will stay at home and rest instead of travelling to face Napoli.
The 36-year-old Portuguese forward spent six seasons at United between 2003-09, winning eight major trophies.